Skip to main content
. 1999 Aug 17;96(17):9637–9641. doi: 10.1073/pnas.96.17.9637

Figure 3.

Figure 3

(A) Immunoblot of pRb, proliferating cell nuclear antigen, and Id-1 expression in Id-1 transfectants. Neo, vector-transfected HFKs 1 month after transfection; Id-1/1, Id-1-transfected HFKs 1 month after transfection; Id-1/6, Id-1-transfected HFKs 6 months after transfection. Actin panel is used as a loading control. (B) Immunoblot of p53 expression in response to DNA damage by actinomycin D for 24 and 72 hr. Neo cells are vector-transfected HFKs 1 month after transfection, Id-1/1 cells are Id-1-transfected HFKs 1 month after transfection, Id-1/6 cells are Id-1-transfected HFKs 6 months after transfection, and 1321 cells are HPV 16 E6/E7-immortalized HFKs. (C) Fluorescence-activated cell sorter analysis of Id-1-immortalized HFK response to DNA damage. Cells were exposed to 2.5 nM actinomycin D for 48 hr, and cell cycle profiles were determined by propidium iodide staining. Neo cells are vector-transfected HFKs, Id-1/6 are Id-1-transfected HFKs 6 months after transfection, and 1321 cells are HPV 16 E6/E7 immortalized HFKs.