Figure 2.
Analysis of PlA2β3 synthesis in transduced promegakaryocytic (Dami) and epithelial (CFT1) cell lines. Immunoblot shows PlA2β3 synthesis in Dami but not CFT1 cells. After cells were transduced with −889PlA2β3, 800 μg of each cell lysate (7.0 × 106 cells) was immunoprecipitated with a β3-specific mAb (AP3). The complexed proteins were separated on a nonreduced SDS/ 8% PAGE gel. PlA2β3 was detected (arrow) by using human anti-PlA2 alloimmune serum and, chemiluminescence. Control represents untransduced cells and LacZ are cells transduced with a retroviral vector encoding β-gal. Molecular mass markers are shown in kDa (left).