Figure 4.

Anti-TGF-β antibodies suppress the stimulation of collagen lattice contraction induced by the eosinophil. Dermal fibroblasts were seeded in collagen lattices (100,000 cells per lattice) and incubated in DMEM + 0.5% FCS alone (solid lozenges) or supplemented with the eosinophil sonicate (10 eosinophils per lattice, ■) or with the eosinophil sonicate preincubated with rabbit anti-TGF-β antibodies (crosses). Open triangles are controls consisting of the eosinophil sonicate preincubated with nonimmune rabbit IgG. Each set of data is the mean of four experiments. SDs were less than 10% of the mean value in every case and were not reported as error bars on the graph for better clarity.