Figure 2.

Effect of PMA on contact formation during wound healing. Contact formation by control (A) or PMA-treated cells (B and C) was documented by using video–differential interference contrast microscopy. B and C represent images of the same cells taken with a 16-min interval. During contact formation in control IAR-2 cells (A), free cell edges adjacent to the contact zone appeared stretched and contained few lamellipodia. Edges of PMA-treated cells contained numerous lamellipodia, which continued to form even after the establishment of the initial cell–cell contact (B). A new leading lamella (arrow) formed at the free edge of a PMA-treated cell resulting in cell reorientation even after contact expansion and increased cell–cell overlapping (C). (Bar = 10 μm.)