Figure 5.

Figf/Vegf-D-induced cell proliferation and chemotactic activity. (A and B) Proliferative effects of Figf/Vegf-D were assayed on HUVECs and KS-IMM cells as indicated. Experiments were performed in medium containing 1% FCS. After 72 h cells were enumerated by using a Coulter counter and values represent the mean (±SEM) of triplicate samples. (C and D) Cells were seeded in the upper wells of a 48-well micro chemotaxis Boyden chamber and incubated for 7 h at 37°C in medium containing 1% FCS. The lower wells contained the indicated concentrations of Figf/VEGF-D. Cells migrating through a polycarbonate membrane with a pore size of 5 μm were quantified by staining the cells with Giemsa solution and counting was performed on a light microscope of five high-power fields (×100). The results are expressed as the mean ± 1 SD of three independent experiments performed in triplicate.