Figure 1.
Northern blot analysis of Bin1 and c-Myc in melanoma cell lines. Fifteen micrograms of total cytoplasmic RNA from each cell line was analyzed by Northern blotting and hybridization with 32P-labeled human Bin1 or c-Myc cDNA probes. (A) Bin1. Arrows indicate the Bin1 mRNAs, and dots indicate the position of 28S and 18S ribosomal RNAs. (Lower) Ethidium bromide-stained 28S RNA is shown as a loading control. (B) c-Myc. MCF7 and ZR75–1 are breast carcinoma cell lines that overexpress c-Myc and that are provided for comparison with the melanoma lines. (Lower) β-Actin hybridization to the same blot is shown as a loading control.