Figure 2.

Nonphysiological splicing of brain-specific Bin1 exon 12A in melanoma. Splice isoforms expressed in fetal melanocytes and melanoma cell lines were identified by RT-PCR. Results from DNA sequencing of the RT-PCR products is presented in Table 1. (Upper) RT-PCR products derived from the 3′ region of Bin1 RNA in which splice variations occur were fractionated on agarose gels and stained with ethidium bromide. LNCaP is a control that identifies the two ubiquitous isoforms found all in nonneuronal cells (−10+13 and −10−13 isoforms), which also are expressed in fetal melanocytes (FM1036). Plasmid cDNA clones of different splice isoforms also were used as template controls (7). The arrowhead identifies 12A isoforms expressed in melanoma cell lines. (Lower) Cartoons illustrate splice isoforms identified (exons numbered), with arrows indicating the position of the primers used for the RT-PCR (Upper).