Table 1.

Structure of Bin1 RNA in melanoma cells

Cell line	Cell type	Sequence	Exon 12A splicing*	Level of 12A protein	Susceptibility to ectopic Bin1
FM 1036	Melanocyte	WT	−	−	−
WM 35	RGP	Five muts	+	+	++
WM 39	RGP	ND	ND	ND	ND
WM 164	Met	WT*†	+	−	−
WM 239A	Met	WT*†	+	+++	+++
WM 793	VGP	WT	+	++	+++
WM 983A	VGP	E180K	+	++	++
WM 983C‡	Met	E180K	+	ND	ND
WM 1341D	Met	D146E	+	+/−	−

RT-PCR products spanning the entire coding region of Bin1 mRNA (7) were subcloned, and their DNA sequence was determined and compared with wild-type Bin1. For comparison, the last column presents the relative level of susceptibility of each cell line to programmed cell death by ectopic nonneuronal Bin1 isoform, illustrating the approximate correlation between relative level of the 12A splice isoform and susceptibility to death by ectopic Bin1. WT, wild type; Met, metastatic melanoma; muts, mutations; ND, not determined. 

^*As detected by RT-PCR. 

^†Aberrant 3′ splice product detected by RT-PCR but not by Northern blot analysis. 

^‡Metastatic derivative of WM983A.