Table 2.
Fraction of sub-G1 phase DNA detected by flow cytometry in cells infected with induced Bin1 adenovirus
| Cell line | cre only (Ad-cre + Ad-vect) | Uninduced Bin1 (Ad-vect + Ad-MABin1) | Induced Bin1 (Ad-cre + Ad-MABin1) | Level of 12A protein |
|---|---|---|---|---|
| WM 35* | 1.6 | 3.5* | 15.2 | + |
| WM 164* | 6.0 | 4.7* | 5.7 | − |
| WM 239A* | 6.3 | 12.8* | 20.9 | +++ |
| WM 793* | 6.1 | 10.7* | 58.8 | ++ |
| CM 983A | 3.5 | 3.7 | 10.3 | ++ |
| WM 1341D | 5.9 | 4.1 | 4.2 | +/− |
Cells were infected with the two adenoviral vectors indicated at 100 moi each and processed 48 hr later for propidium iodide staining and flow cytometry as described in Materials and Methods. The proportion of each cell population that was scored in the sub-G1 phase is shown. For comparison, the relative level of endogenous expression of 12A-containing species in each line is shown, illustrating the correlation with susceptibility to Bin1-induced DNA degradation.
*
Exhibits leaky expression (see Fig. 5).