Figure 1.
UVA, UVB or UVC induces phosphorylation of H2AX
(A) JB6 cells were exposed to (A) UVA, (B) UVB, or (C) UVC and histones were extracted as described in “Experimental Procedures” after the incubation time indicated (left panels) or after 60 min following indicated doses of UVA (right panels). Cells not exposed to UV served as negative controls (−). For all experiments, histones were resolved by 15% SDS-PAGE followed by western analysis with antibodies against γH2AX or total H2A.