Table 1.
Summary of the olfactory receptor genes expressed in the septal organ confirmed by different means
|
|
Gene name |
NBT—BCIP-positive cells per section (mean ± SD) |
Percentage in OMP-positive cells (mean ± SD) |
Fold-change (SO vs MOE) in genechips |
cDNA cloning |
Specific RT-PCR |
|||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Category I | MOR256-3 | 49.8 ± 0.9%; n = 12 | 31.1 | + | |||||||
| Category II | MOR244-3 | 35.7 ± 12.1; n = 18 | 12.2 ± 0.8; n = 10 | 26.3 | + | ||||||
| MOR0-2a | 23.9 ± 8.8; n = 16 | 8.0 | 7.2 | + | |||||||
| MOR235-1 | 19.4 ± 8.6; n = 17 | 6.5 | 3.2 | ++++ | |||||||
| MOR160-2 | 14.2 ± 4.1; n = 5 | 4.7 | - | +++ | |||||||
| MOR122-1 | 10.9 ± 4.6; n = 17 | 3.7 | - | ++++ | |||||||
| MOR160-5 | 8.6 ± 7.5; n = 18 | 2.9 | 2.9 | + | |||||||
| MOR236-1 | 7.8 ± 3.3; n = 12 | 2.6 | - | ++ | |||||||
| Category III | MOR244-2 | 3.8 ± 1.8; n = 9 | 1.3 | 1.1 | ++ | ||||||
| MOR271-1 | 1.3 ± 2.1; n = 14 | 0.4 | 1.0 | +++ | |||||||
| MOR232-2 | 0.7 ± 0.8; n = 10 | 0.2 | - | +++ | |||||||
| Category IV | The following antisense RNA probes stained less than one cell per section, but their existence in the septal organ was confirmed by other means: MOR40-12 and MOR31-12 by genechips (both showed 2.6-fold increment as compared to the MOE) and specific RT-PCR; MOR13-6, MOR257-1, MOR103-15, MOR171-2, MOR279-2, MOR174-13 and MOR225-2 by cDNA cloning. | ||||||||||
| Category V |
The following sequences were encountered only in one RNA sample in cDNA cloning, but their existence was not verified by in situ hybridization. MOR3-1; MOR9-2; MOR13-4; MOR14-4; MOR24-2; MOR29-1; MOR30-3; MOR31-4; MOR33-1; MOR33-2; MOR103-16; MOR132-1; MOR135-9; MOR135-13; MOR135-19; MOR135-20; MOR160-1; MOR161-5; MOR163-1; MOR164-1; MOR164-3; MOR171-6; MOR171-22; MOR171-23; MOR171-24; MOR171-43; MOR171-44; MOR172-2; MOR174-10; MOR174-12; MOR174-13; MOR174-15P; MOR174-18; MOR179-3; MOR185-7; MOR185-8; MOR187-1; MOR187-2; MOR188-3; MOR194-1; MOR203-1; MOR204-1; MOR204-23; MOR206-3; MOR206-4; MOR206-6; MOR208-5P; MOR209-1; MOR211-1; MOR224-4; MOR225-6P; MOR225-8P; MOR231-11; MOR232-5; MOR239-5; MOR256-12; MOR257-2; MOR262-5; MOR264-4; MOR274-1; MOR276-1; MOR277-1; MOR281-1; MOR284-1. |
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The “NBT-BCIP positive cells” were counted and averaged (mean ± SD) from all the septal organ sections (n) stained by each RNA probe. NBT, Nitroblue tetrazolium; BCIP, 5-bromo-4-chloro-3-indolyl phosphate. The “percentage in OMP-positive cells” for MOR256-3 and MOR244-3 was obtained from double in situ hybridization, and the rest was estimated according to the MOR244-3 data generated in the two ways (see Results). The “fold-change (SO vs MOE) in genechips” was averaged from the two experiments, and a dash indicates “not detected.” The number of the plus signs under “cDNA cloning” indicates the number of RNA samples (of four) from which each sequence was encountered.