Table 3.
Average nucleotide substitutions per synonymous site (d̄s) and per nonsynonymous site (d̄N) at the rabbit Ig VH loci
| Genes | No. of sequences | CDRs
|
FRs
|
||||
|---|---|---|---|---|---|---|---|
| d̄S | d̄N | d̄N/d̄S | d̄S | d̄N | d̄N/d̄S | ||
| VH1 alleles | 3 | 0.17 ± 0.11 | 0.16 ± 0.05 | 0.94 | 0.11 ± 0.04 | 0.14 ± 0.03 | 1.3 |
| Functional genes | 16 | 0.23 ± 0.09 | 0.21 ± 0.04 | 0.91 | 0.13 ± 0.03 | 0.19 ± 0.02 | 1.4 |
| Pseudogenes | 9 | 0.23 ± 0.09 | 0.23 ± 0.04 | 1.0 | 0.13 ± 0.03 | 0.20 ± 0.02 | 1.5* |
d̄N and d̄S were estimated by the method of Nei and Gojobori (19), and the standard errors were computed by the method of Nei and Jin (42). All computations were done with the sequence data after complete deletion, which excluded all sites having one or more alignment gaps.
*d̄N is significantly higher than d̄S at the 5% level (P < 0.05).