TABLE 3.

Sequence matches for the bands excised from the DGGE gels containing sheep rumen samples^a

Amplicon	Band	Accession no.	Most similar sequence (accession no.)	% Identityb	Occurrence
V1-V2	1	EF442682	Methanobrevibacter sp. strain NT7 (AJ009959)	100	All samples
	2	EF442683	Methanobrevibacter sp. strain AbM4 (AJ550156)	98	All samples except F-3-D14 and F-4-D14
	3	EF442684	Methanobrevibacter sp. strain AbM4 (AJ550156)	97	All samples except F-3-D14 and F-4-D14
V3	2	EF442689	Methanobrevibacter sp. strain AbM4 (AJ550156)	99-100c	All samples except F-3-D14 and F-4-D14
	4	EF442690	Methanobrevibacter smithii PS (AY196669)	100	All samples
	5	EF442691	Methanobrevibacter runimantum M1 (AY196666)	99	All samples
	6	EF442692	Methanobrevibacter woesei GS (U55237)	100	All samples
	7	EF442693	Methanobrevibacter smithii PS (AY196669)	100	All samples
	8	EF442694	Methanosphaera stadtmanae MCB-3 (AY196684)	96	F-3-D14 and F-4-D14
V3-V4	1	EF442685	Methanobrevibacter sp. strain AbM4 (AJ550156)	98	All samples except F-3-D14 and F-4-D14
	2	EF442686	Methanobrevibacter thaueri CW (U55236)	100	C-2-D23
	3	EF442687	Methanobrevibacter runimantum NY-1 (AB034183)	99	All samples
	4	EF442688	Methanobrevibacter smithii PS (AY196669)	96	C-1-D14, C-2-D14, F-3-D23, F-4-D23

^aSee Fig. 2.

^bRoundup values and ambiguous bases were considered the same.

^cThe sequences of bands 1 and 3 are very similar to the sequence of band 2.