FIG. 3.

Bioassay done with CCB grown with S. entomophila Mor4.1 showing the AFE and mortality in third-instar P. blanchardi larvae. Broth samples cultured with S. entomophila Mor4.1 for 48 h and 72 h were evaluated (n = 14). Control larvae were fed with nutrient broth agar containing bovine serum albumin. Broth samples from S. entomophila Mor4.1 (48-h culture) were treated with proteinase K (PK), added to nutrient broth agar, and fed to the larvae. The AFE was recorded after 12 days and the mortality after 30 days from the beginning of the test. The lack of significant difference is indicated by the same letter above the bars (χ² P < 0.01 for the AFE at 48 h; P < 0.05 for the AFE at 72 h and for mortality at 48 h).