TABLE 3.

Detection of horizontal gene transfer events in MLST data set^a

HGT event	ST(s) involved in HGT event	Nb	PPSTc		Ad	Breakpoint		Gene(s)e	Pf
			M	m		Start	End
1	82	1	217	274	6	255	876	glpF-gmk	≤4.072 × 10−12
2	21	1	222	59	5	381	876	gmk	≤1.707 × 10−14
3	207	1	275	112	6	885	1245	ilvD	≤4.656 × 10−7
4	125	1	250	127	5	933	1245	ilvD	≤8.296 × 10−5
5	271, 277, 279, 280, 283, 284	6	196	208	6	882	1251	ilvD	≤9.370 × 10−15
6	273, 282, 285	3	196	208	6	882	1251	ilvD	≤1.660 × 10−9
7	124, 253	2	153	102	4	1101	1266	ilvD	≤4.179 × 10−6
8	80	1	59	140	6	879	2031	ilvD-pur	≤1.890 × 10−13
9	12, 16, 23, 56, 61, 197, 257, 203, 265	9	204	102	3	885	1749	ilvD-pur	≤2.125 × 10−3
10	148	1	17	11	6	879	1302	ilvD-pta	≤2.613 × 10−17
11	36	1	272	5	6	885	1302	ilvD-pta	≤1.203 × 10−12
12	173	1	76	232	3	1302	1674	pta	≤8.100 × 10−8
13	38, 78, 103, 123, 132, 369	6	266	84	5	1531	2031	pta-pur	≤8.880 × 10−3
14	47, 171, 371, 374	4	266	84	5	1551	2031	pta-pur	≤1.843 × 10−2
15	28, 79, 188	3	266	84	5	1551	2031	pta-pur	≤1.453 × 10−4
16	6	1	266	84		1551	2031	pta-pur	≤7.535 × 10−3
17	45, 91, 121, 122, 140, 141, 181, 182, 183, 185, 205	11	266	84	5	1767	1914	pur	≤2.304 × 10−5
18	1, 2, 3, 107, 110, 112, 130, 134, 135, 145	10	62	229	6	1693	1890	pur	≤1.535 × 10−9
19	5, 7, 32, 90, 92, 120, 127, 169, 172, 180, 211, 219, 234	13	144	373	6	1710	2031	pur	≤4.752 × 10−4
20	26, 31, 165	3	144	373	6	1710	2031	pur	≤6.820 × 10−8
21	105	1	164	210	4	1740	1911	pur	≤1.028 × 10−4
22	230	1	203	207	6	1687	2031	pur	≤1.933 × 10−11
23	264	1	16	235	5	1693	2011	pur	≤1.640 × 10−13
24	193	1	213	248	6	2034	2391	pycA	≤3.809 × 10−12
25	110, 214	2	106	378	6	2061	2697	pycA-tpi	≤2.557 × 10−3

^aUnderlined values indicate possible misidentifications of breakpoint positions. Boldface values indicate STs for which the toxin distribution is available. Only HGT events detected by at least three methods and having both potential ST parents present in the MLST data set are listed.

^bN, number of STs subject to HGT.

^cPPST, potential parent ST; M, major potential parent; m, minor potential parent.

^dA, number of different algorithms supporting the HGT event.

^eThat is, the gene fragments involved in the HGT event.

^fThe P value was obtained after multiple-comparison correction. Because each HGT event was supported by at least three algorithms, Table 3 lists only the highest P value obtained for the specific HGT (i.e., the least statistically significant). Values listed below the breakpoint's start and end refer to the positions of the MLST data set of the first ST recorded for the specific HGT event.