FIG. 4.

(A) Schematic representation of the strategy used to map the epitope on the MOMP. Lines represent fragments of MOMP that were constructed, and the flanking numbers indicate the amino acid positions. Epitope mapping was carried out in two stages. In stage I, six overlapping fragments were created, and all reacted with MAb5C4. In stage II, five fragments were created, but only three fragments reacted with MAb5C4. The amino acid sequence of the shortest overlapping region, 13 amino acids in length, is also displayed. +, positive reaction; −, negative reaction. (B) Alignment of a partial sequence of the MOMP containing the amino acids required for binding by MAb5C4. The amino acids in bold letters are conserved among C. jejuni but divergent in other Campylobacter species. Asterisk, identical amino acids; colon, conserved amino acid substitutions; period, semiconserved amino acid substitutions. The MOMP gene sequences determined in this study and deposited in GenBank are indicated (†).