TABLE 2.
Neutralization to stop sporicidal activity
| Species tested | Sporicide (concn) | Neutralizer | Log10 CFU recovered
|
||
|---|---|---|---|---|---|
| Control carrier | Neutralizer + sporicide | Neutralizer alone | |||
| B. licheniformis (ATCC 14580) | Sodium hypochlorite (5,000 ppm FAC) | 1% sodium thiosulfate + 0.1% Tween 80 in saline | 6.75 | 6.71 | 6.80 |
| B. atrophaeus (ATCC 51189) | Sodium hypochlorite (5,000 ppm FAC) | 1% sodium thiosulfate + 0.1% Tween 80 in saline | 6.90 | 6.98 | 6.86 |
| B. subtilis (ATCC 6051) | Sodium hypochlorite (5,000 ppm FAC) | 1% sodium thiosulfate + 0.1% Tween 80 in saline | 6.60 | 6.50 | 6.53 |
| B. atrophaeus (ATCC 51189) | AHP (70,000 ppm) | Letheen broth + 1% sodium thiosulfate | 6.71 | 6.71 | 6.96 |
| B. licheniformis (ATCC 14580) | AHP (70,000 ppm) | Letheen broth + 1% sodium thiosulfate | 7.05 | 6.98 | 7.03 |
| B. subtilis (ATCC 6051) | AHP (70,000 ppm) | Letheen broth + 1% sodium thiosulfate | 6.69 | 6.59 | 6.65 |
| B. anthracis (ATCC ADRI 9610) | AHP (70,000 ppm) | Letheen broth + 1% sodium thiosulfate | 6.49 | 6.52 | 6.56 |
| B. subtilis (ATCC 6051) | PAA (3,000 ppm) | 1% sodium thiosulfate + 0.1% Tween 80 in saline | 6.43 | 6.41 | 6.36 |
| B. licheniformis (ATCC 14580) | PAA (3,000 ppm) | 1% sodium thiosulfate + 0.1% Tween 80 in saline | 6.84 | 6.80 | 6.82 |
| B. atrophaeus (ATCC 51189) | PAA (3,000 ppm) | 1% sodium thiosulfate + 0.1% Tween 80 in saline | 6.63 | 6.63 | 6.65 |
| B. atrophaeus (ATCC 51189) | Chlorine dioxide (1,000 ppm) | 1% sodium thiosulfate + 0.1% Tween 80 in saline | 6.68 | 6.66 | 6.73 |
| B. subtilis (ATCC 6051) | Chlorine dioxide (1,000 ppm) | 1% sodium thiosulfate + 0.1% Tween 80 in saline | 6.25 | 6.36 | 6.32 |