FIG. 1.

DGGE profiles of bacteria present on the hindgut walls of D. albohirtum third-instar larvae collected in 2005 from regions approximately 200 km apart by using general primers targeting the 16S rRNA gene. Sampling areas were the Cairns region (lanes 1 and 2), Tully region site 1 (lanes 5 to 7) and site 2 (lanes 8 to 10), Ayr region site 1 (lanes 11 to 13) and site 2 (lanes 14 to 16), and the Mackay region (lanes 19 and 20). Lanes 4 and 17 are ladders. Lanes 3 and 18 contain samples from hindguts of humus-feeding Dasygnathus sp. larvae found in the same root bowls as the larva samples in lanes 2 and 19, respectively. Letters to the left of the lanes indicate bands that were selected for sequencing. Sequence Da-5 was isolated from bands a and b. Sequence Da-2 was isolated from bands e to g, and Da-3 was isolated from band h. Dominant bands are presented in the phylogenetic tree (Fig. 2).