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. 2007 Dec 7;74(3):745–752. doi: 10.1128/AEM.01843-07

FIG. 1.

FIG. 1.

Diagram of bovine-specific (A) and fecal indicator (B) plasmid DNA composite IAC constructs. The bovine-specific IAC (152 bp) consists of a VIC-labeled universal probe binding site flanked by primer sequences for CowM2 (118 bp) and CowM3 (131 bp) qPCR assays. The fecal indicator IAC (261 bp) contains a binding site for a TET-labeled universal probe and primer sequences for GB342 (160 bp) (28), Btheta (146 bp) (Blackwood and Noble, presented at the 105th General Meeting of the American Society for Microbiology), and Entero1 (106 bp) (21) qPCR assays (Table 1).