TABLE 1.
Oligonucleotides, primers, and probes
| Assay | Primer and probe sequences (5′→3′) | Size (bp) | Reference or source |
|---|---|---|---|
| Btheta | Forward, CGTTCCATTAGGCAGTTGGT; reverse, ACACGGTCCAAACTCCTACG; probe, FAM-CTGAGAGGAAGGTCCCCCACATTGGA-TAMRA | 110 | Blackwood and Noblea |
| GB342 | Forward, GGGGTTCTGAGAGGAAGGT; reverse, AGTAGCGTGAAGGATGACGG; probe, FAM-CAATATTCCTCACTGCTGCCTCCCGTA-TAMRA | 129 | 28 |
| Entero1 | Forward, AGAAATTCCAAACGAACTTG; reverse, AATGATGGAGGTAGAGCACTGA; probe, FAM-TGGTTCTCTCCGAAATAGCTTTAGGGCTA-TAMRA | 92 | 21 |
| CowM2 | CowM2F, CGGCCAAATACTCCTGATCGT; CowM2R, GCTTGTTGCGTTCCTTGAGATAAT; probe, FAM-AGGCACCTATGTCCTTTACCTCATCAACTACAGACA-TAMRA | 92 | This study |
| CowM3 | CowM3F, CCTCTAATGGAAAATGGATGGTATCT; CowM3R, CCATACTTCGCCTGCTAATACCTT; probe, FAM-TTATGCATTGAGCATCGAGGCC-TAMRA | 122 | This study |
| Cow IAC | Frag1, CGGCCAAATACTCCTGATCGTCCTCTAATGGAAAATGGATGGTATCTATTACTCATAGGCACTAGGAACAGGCGGCGACGAAACTACAGACAAAATTATCTCAAGAACG; Frag2, CCATACTTCGCCTGCTAATACCTTAAGGACATAGGCTTGTTGCGTTCCTTGAGATAATTTTGTCTGTAGTTTCGTCGCCGCCTGTTCCTAGTGCCTATGAGTAATAGATA; probe, VIC-TAGGAACAGGCGGCGACGA-TAMRAb | 152 | 42; this study |
a
Presented at the 105th General Meeting of the American Society for Microbiology.
b
The TaqMan probe was modified from the previously reported UT probe (42).