TABLE 1.

General and statistical data for calculated ratios of precursor 16S rRNA to mature 16S rRNA

Primer used	Culture temp (°C)	Timeb (min)	Mean pre-16S:16S ratio	SD	No. of samples	COVc (%)
Eub338	25	0	0.073	0.001	4	2.0
		10	0.145	0.002	3	1.0
		20	0.270	0.021	4	7.9
	30	0	0.126	0.014	3	11.0
		10	0.265	0.013	4	4.7
		30	0.571	0.011	3	1.9
	35	0	0.266	0.006	3	2.2
		20	0.513	0.010	5	2.0
		30	0.669	0.036	3	5.4
	30a	0	0.104	0.014	3	13.4
		10	0.104	0.019	3	17.8
		20	0.093	0.012	3	12.6
Acin0659	25	0	0.136	0.005	4	3.6
		10	0.272	0.027	3	9.8
		20	0.390	0.01	3	2.6
	30	0	0.105	0.008	5	8.0
		10	0.195	0.006	4	10.6
		20	0.265	0.002	3	2.3
	35	0	0.473	0.025	5	5.3
		10	0.606	0.037	5	6.1
		30	0.957	0.008	3	0.9
	30a	0	0.091	0.001	3	0.9
		10	0.094	0.003	3	2.8
		30	0.088	0.007	4	8.2

^aStationary-phase culture.

^bTime of exposure to chloramphenicol.

^cCOV, coefficient of variance.