TABLE 2.
Specific rates of ribosome synthesis and specific growth rates as calculated by RT-RiboSyn and spectrophotometry as well as percent differences between the measurements
Culture temp (°C) | Result for indicated methoda
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Spectro photometry
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RT-RiboSyn
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Eub335
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Acin0659
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μ (h−1) | R2 | r (h−1) | R2 | % Diff | r (h−1) | R2 | % Diff | |
25 | 0.381 | 0.811 | 0.387 | 0.971 | 1.6 | 0.482 | 0.998 | 21.0 |
30 | 0.550 | 0.988 | 0.611 | 0.999 | 10.0 | 0.532 | 0.926 | −3.3 |
35 | 0.562 | 0.925 | 0.545 | 0.996 | −3.1 | 0.665 | 0.994 | 15.5 |
30b | 0.007 | 0.476 | −0.017 | 0.706 | NA | −0.004 | 0.358 | NA |
r, specific rate of ribosome synthesis; μ, specific growth rate; % diff, percent difference between the measurements; NA, not applicable. The R2 values of the linear regression are included.
Stationary-phase culture.