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Transcription of bsh and lmo2085 in BHI is σB dependent. mRNA was extracted from stationary-phase (A) and exponential-phase (OD600, ∼0.4) (B) L. monocytogenes 10403S wild-type (sigB +) and ΔsigB (sigB −) cells in the presence (NaCl +) or in the absence (NaCl −) of 0.5 M NaCl. The numbers in parentheses indicate the numbers of PCR cycles to which the cDNA templates were subjected. The results are representative results of at least three replicates.
