TABLE 2.
Proteins identified in stationary-phase extracts from BHI and BHIS analyzed by 2-DGE
| Protein | Homologous protein (% identity/% similarity)a | Microorganism with homologous protein | Mol wt (predicted/observed) | pI (predicted/observed) | % Coverage | % Matched peptides | Scoreb |
|---|---|---|---|---|---|---|---|
| Proteins expressed in a σB-dependent manner | |||||||
| Lmo0539 | Tagatose-1,6-diphosphate aldolase (44/61) | Staphylococcus aureus | 37.8/39.0 | 4.9/4.9 | 45 | 54 | 824 |
| Lmo0796 | YceI (44/61) | Escherichia coli | 19.3/19.4 | 4.6/4.4 | 60 | 69 | 102 |
| Lmo1830 | Short-chain dehydrogenase (47/67) | Pseudomonas aeruginosa | 20.9/22.0 | 5.9/6.2 | 60 | 85 | 124 |
| Lmo2391 | YhfK (42/61) | Bacillus subtilis | 22.7/25.0 | 6.0/6.5 | 65 | 62 | 110 |
| Proteins expressed in a σB-dependent manner in the presence of salt | |||||||
| Lmo0913 | Succinate semialdehyde dehydrogenase (48/67) | Bacillus subtilis | 53.2/55.0 | 5.8/6.4 | 23 | 26 | 78 |
| Lmo0913a | Succinate semialdehyde dehydrogenase (48/67) | Bacillus subtilis | 53.2/55.0 | 5.8/6.3 | 49 | 64 | 999 |
| Lmo2696 | Dihydroxyacetone kinase (50/68) | Lactococcus lactis | 21.5/20.0 | 5.1/5.1 | 36 | 23 | 77 |
| Lmo2748 | YdaG (42/68) | Bacillus subtilis | 15.7/10.0 | 4.6/4.0 | 54 | 78 | 81 |
| Proteins expressed in a growth phase-dependent manner | |||||||
| Fri | Nonheme iron-binding ferritin (100/100) | Listeria monocytogenes | 16.0/15.5 | 4.7/5.0 | 69 | 15 | 77 |
| Lmo1538 | Glycerol kinase (74/88) | Bacillus subtilis | 55.0/60.0 | 4.9/4.9 | 28 | 24 | 88 |
| Lmo2101a | Pyridoxine biosynthesis protein (83/92) | Bacillus subtilis | 31.8/32.0 | 5.3/4.9 | 34 | 24 | 78 |
| Lmo2101b | Pyridoxine biosynthesis protein (83/92) | Bacillus subtilis | 31.8/32.0 | 5.3/5.0 | 50 | 29 | 107 |
| Lmo2101c | Pyridoxine biosynthesis protein (83/92) | Bacillus subtilis | 31.8/32.0 | 5.3/5.2 | 29 | 27 | 104 |
a
The identity and similarity values were obtained by performing a protein-protein BLAST search on the NCBI website (http://www.ncbi.nlm.nih.gov/BLAST/).
b
The score was derived from ion scores that were equal to −10×log(P), where P is the probability that the observed match is a random event.