TABLE 2.
Relative phylum-level compositions of depth-resolved bacterial 16S rRNA gene clone libraries and selected genus- or lineage-specific clone frequencies
| Phylogenetic affiliationb | % of clones in indicated library at depth (bgs) ofc:
|
T-RF length (bp)a
|
||||
|---|---|---|---|---|---|---|
| D10
|
D12
|
D15
|
D25
|
Predicted | Measured | |
| 6.3 m | 6.8 m | 7.6 m | 11.7 m | |||
| Alphaproteobacteria | 31 | 3 | NA | |||
| Methylocystis related | 3 | NA | ||||
| Betaproteobacteria | 26 | 19 | 14 | 123/492 | 120/492 | |
| Thiobacillus related | 6 | 6 | NA | |||
| Gallionella related | 6 | 6 | 123/125 | 120/122 | ||
| Gammaproteobacteria | 9 | NA | ||||
| Beggiatoa related | 6 | 138 | 135 | |||
| Deltaproteobacteria | 26 | 16 | 11 | NA | ||
| Desulfocapsa related | 6 | 162 | 159 | |||
| Geobacter related | 19 | 132 | 130 | |||
| Syntrophus related | 7 | 6 | 127/509 | 123/509 | ||
| Desulfobacterium related | 6 | 166 | 164 | |||
| Bacteroidetes | 6 | 14 | 91 | 87 | ||
| Nitrospirae | 3 | 7 | 8 | NA | ||
| Magnetobacterium related | 5 | 8 | 290 | 285 | ||
| Bacilli | 10 | 8 | 137 | 133 | ||
| Clostridia | 14 | 29 | 2 | 3 | NA | |
| Sedimentibacter related | 19 | 280 | 279 | |||
| Uncultured Peptococcaceae | 6 | 282 | 281 | |||
| Desulfosporosinus related | 3 | 227 | 225 | |||
| Desulfotomaculum related | 3 | 214 | ND | |||
| Actinobacteria | 3 | 3 | 16 | 11 | NA | |
| Rubrobacter related | 7 | 6 | 131/162 | 128/160 | ||
| Chloroflexi | 3 | 23 | 17 | NA | ||
| Uncultured I | 14 | 518 | 518 | |||
| Uncultured II | 3 | 21 | 3 | 373/454/523 | 373/453/522 | |
| Dehalococcoides related | 2 | NA | ||||
| TM6 | 9 | NA | ||||
| OP5 | 7 | 230 | 224 | |||
| OP10 | 7 | 3 | NA | |||
| TM7/OP11 | 3 | 9 | NA | |||
| Others | 3 | 3 | 12 | 8 | NA | |
Characteristic T-RF lengths (bp) predicted from the sequence data for all or a major portion of the clones of a given affiliation are indicated together with T-RF lengths actually measured in T-RFLP analysis. Values separated by a slash indicate more than one characteristic T-RF for a lineage. NA, not applicable; ND, not determined.
The naming of phyla without cultivated representatives is in accordance to reference 44.
The libraries at 6.3, 6.8, 7.6, and 11.7 m contained 35, 31, 43, and 36 clones, respectively. Division-level percentages (given in bold) include the genus- or lineage-specific percentages (nonbold).