Table 2. .
Sequencing Primers and PCR Conditions for the CD96 and ZBED2 Genes
| Sequence(5′→3′) |
|||||
| Primer Name | Forward | Reverse | Tma (°C) |
MgCl2 (mM) |
Size (bp) |
| hCD96 ex1 | CAACTGCTCTGCGTGATATC | ACCCTTAGTAATGATTTGTCCT | 60 | 2.5 | 540 |
| hCD96 ex2 | CCTAAAGCAGCCAGGGAGAAA | ATGCTGAGCACCAAGCCTAAC | 58 | 1.25 | 657 |
| hCD96 ex3 | GAGGACAGATGAATCCCTATAC | ATAGACTCAGAGGCTTGCCTG | 60 | 1.8 | 424 |
| hCD96 ex4 | CAGACTTGCCAGTGCTGAGT | GGATGGACTAAGGTAGACTTC | 60 | 1.8 | 380 |
| hCD96 ex5 | GTAAATGAATCAGTGCTTGTCGA | GTATCCAGGGAAACAGACTCC | 62 | 2.5 | 429 |
| hCD96 ex6 | TCTGTATTCCCATGAAACTGTAG | TATGCAACCTGACACACCTTAC | 60 | 1.8 | 367 |
| hCD96 ex7 | CATCTCTATAGGAGATAGCCCA | ACACTCCACCCCCTTGGAAG | 58 | 1.25 | 472 |
| hCD96 ex8 | TTGATCATGCCATGCCTTGGC | TTTCACTGGAGTCCTACTTGTC | 58 | 1.25 | 446 |
| hCD96 ex9 | GCTGCCTAGTTTCCAGGCCA | ATGGGCAAGTTAATGTGACGTG | 58 | 1.25 | 485 |
| hCD96 ex10 | GGCTGTTCACTAAGATTCTTTCC | TAGTCACCGCAGAGTAACCCA | 58 | 1.25 | 343 |
| hCD96 ex11 | GCCAGCTAGTGTTCCTGCATA | GTCCATGGGTGTAGTCTCAGA | 60 | 1.8 | 386 |
| hCD96 ex12 | CAAGAATCCCTTCAACTCCCAC | TATATCTATCTGAGGCTGGCTTC | 62 | 1.8 | 355 |
| hCD96 ex13 | CAAATCTCAGGATCCCAGCCT | TTGACCCTGACAACACCTTATC | 62 | 1.25 | 499 |
| hCD96 ex14 | GCTTAGACATGCCCACCTCC | CAGCCTGACTAGGCCAATGC | 62 | 1.25 | 488 |
| hCD96 ex15 | TGTGACTAACAGGCACAGGGT | GGTTAAGCTTCAGGCGTTTGG | 58 | 1.25 | 467 |
| hCD96 ex15-2 | GAGAGCCAGAACTACCCAGC | CCACTCCCTACCCCCACTTT | 62 | 1.8 | 372 |
| hZBED2 15 | TGTGGTTCAAATAAGCTTTTGGC | … | 60 | 1.25 | 934 |
| hZBED2 23 | GTTTCGGCCAAGGGTCAGCA | … | … | … | … |
| hZBED2 35 | ACATGATGAGGCGGGAAGACGA | … | 60 | 1.25 | 657 |
| hZBED2 43 | AACAAAATGGAAGGGATGTACTG | … | … | … | … |
a
Annealing temperature.