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. 1999 Aug 17;96(17):9751–9756. doi: 10.1073/pnas.96.17.9751

Figure 2.

Figure 2

Identification of alternative splicing of human Dnmt1 transcript. (A) A 2% agarose gel electrophoresis of RT-PCR products of human K562 RNA. RT-PCR was carried out as described in Materials and Methods by using the PCR primer pair 502–521 and 1,001–981. Lane M, DNA length marker; lane 1, PCR product without RT reaction; lane 2, RT-PCR product. (B) Nucleotide sequences of the two bands, 550 bp and 500 bp, from lane 2 in A. The sequence of the coding strand of the 500-bp fragment from position 607 to 756 is shown together with the corresponding amino acids. Sequence of the inserted 48-bp segment in the 550-bp fragment is aligned with the homologous region of the antisense strand of the Alu consensus sequence described in ref. 33. Note that the 48-bp insertion results in the substitution of proline (P) at codon 149 by arginine (R), as well as the in-frame insertion of another 16 amino acids, serine (S) through alanine (A).