A: Representative Western blot analysis of HSC iNOS. Equal protein loading was confirmed with β-actin antibody. Lane 1: 5.5 mmol/l glucose; lane 2: 5.5 mmol/l glucose + ABA; lane 3: 30 mmol/l glucose; and lane 4: 30 mmol/l glucose + 5 mmol/l ABA. B: Inducible nitric oxide synthase protein content in HSC cultured in 5.5 mmol/l glucose (1), 5.5 mmol/l glucose + ABA (2), 30 mmol/l glucose (3), and 30 mmol/l glucose plus ABA (4). Total iNOS content in cells cultured in 5.5 mmol/l glucose is taken as 100%. Mean ± SE, n = 5 per group. **P < 0.01 vs. cells cultured in 5.5 mmol/l glucose, ##P < 0.01 vs. cells cultured in 30 mmol/l glucose without ABA.