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. Author manuscript; available in PMC: 2008 Feb 4.
Published in final edited form as: Diabetes. 2005 Dec;54(12):3435–3441. doi: 10.2337/diabetes.54.12.3435

FIG. 7.

FIG. 7

A: Representative Western blot analysis of HSC nitrosylated proteins. Equal protein loading was confirmed with β-actin antibody. Lane 1: 5.5 mmol/l glucose; lane 2: 5.5 mmol/l glucose + ABA; lane 3: 30 mmol/l glucose; lane 4: 30 mmol/l glucose + 5 mmol/l ABA; and lane 5: nitrotyrosine positive control. B: Total nitrosylated protein content in HSC cultured in 5.5 mmol/l glucose (1), 5.5 mmol/l glucose + ABA (2), 30 mmol/l glucose (3), and 30 mmol/l glucose plus ABA (4). Total nitrosylated protein content in cells cultured in 5.5 mmol/l glucose is taken as 100%. C: Representative Western blot analysis of HSC poly-(ADP-ribosyl)ated proteins. Equal protein loading was confirmed with β-actin antibody. Lane 1: 5.5 mmol/l glucose; lane 2: 5.5 mmol/l glucose + ABA; lane 3: 30 mmol/l glucose; lane 4: 30 mmol/l glucose + 5 mmol/l ABA; and lane 5: poly(ADP-ribosyl)ated protein positive control. D: Total poly(ADP-ribosyl)ated protein content in HSC cultured in 5.5 mmol/l glucose (1), 5.5 mmol/l glucose + ABA (2), 30 mmol/l glucose (3), and 30 mmol/l glucose + ABA (4). Total poly(ADP-ribosyl)ated protein content in cells cultured in 5.5 mmol/l glucose is taken as 100%. E: Representative Western blot analysis of HSC PARP-1 protein. Equal protein loading was confirmed with β-actin antibody. Lane 1: 5.5 mmol/l glucose; lane 2: 5.5 mmol/l glucose + ABA; lane 3: 30 mmol/l glucose; lane 4: 30 mmol/l glucose + 5 mmol/l ABA; and lane 5: PARP-1 enzyme. F: Total PARP-1 protein content in HSC cultured in 5.5 mmol/l glucose (1), 5.5 mmol/l glucose + ABA (2), 30 mmol/l glucose (3), and 30 mmol/l glucose + ABA (4). Total PARP-1 protein content in cells cultured in 5.5 mmol/l glucose is taken as 100%. In B, D, and F, the results are expressed as mean ± SE, n = 5 per group. **P < 0.01 vs. cells cultured in 5.5 mmol/l glucose; ##P < 0.01 vs. cells cultured in 30 mmol/l glucose.