Table 2.
Effects of cytokines on BDNF and NT3 gene expression: comparison of gene array and QRT-PCR results
| ACCESSION ID | GENE | TREATMENT | FOLD CHANGE | |
| 6 hr array | 6 hr PCR | |||
| Al030286, X67108 | BDNF | Th1 cytokines | -4.19 | -1.14 |
| MM cytokines | no change | 1.09 | ||
| Th2 cytokine | 2.11 | 2.08 | ||
| E03082 | NT3 | Th1 cytokines | -4.15 | -2.5 |
| MM cytokines | -2.71 | -1.09 | ||
| Th2 cytokines | -4.73 | 2.13 | ||
CNS glial cultures were treated with the cytokine mixtures and analyzed by Affymetrix gene array or QRT-PCR, as described in Methods. The gene array values are the averages of 3 experiments; the QRT-PCR values are the averages of 3–5 experiments.