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Assays for HsdR and HsdM polypeptides after treatment with 2-AP. (a) clp+ bacteria (NK301). (b) clpX bacteria (NK304). In the absence of 2-AP (data not shown), the assays for clp+ and clpX bacteria were indistinguishable from those seen in b. EcoKI polyclonal antibody, used in these Western blots, fails to detect HsdS, but detects HsdR and HsdM and some other E. coli proteins.
