Figure 4.

T cells from immunized mice that were expanded in vitro on low doses of 3K peptide stained more intensely and at higher frequency with 3K-IA^b/PESA than cells expanded on high doses of peptide. Eight days after mice were immunized with 50 or 0.5 μg 3Kp, lymph nodes were harvested and cells were cultured for 4.5 days in vitro at five different starting concentrations of 3Kp. Control Eα-IA^b/PESA histograms were matched to and subtracted from the 3K-IA^b/PESA histograms, and a normalized number of the specific, 3K-IA^b/PESA-staining events are depicted in each histogram.