Figure 5.

(A) The increase in CD44hi cells among emigrant T cell populations postexport is gradual and does not indicate repertoire skewing. Donor-derived T cells from congenic thymic grafts were detected in the spleen, lymph nodes, and blood of Ly5.1 recipient mice by using anti-Ly5.2 in conjunction with anti-CD44, anti-CD4, and anti-CD8. A represents the mean proportion of CD44hi-expressing emigrant cells derived from recipient lymph nodes. Between 3 and 10 mice were assessed per group, with error bars representing 1 SD from the mean. B shows the Vβ profile of T lymphocytes (a similar distribution was observed for CD4 and CD8 subsets) derived from the lymph nodes of a mouse grafted for 9 weeks. This profile is representative of profiles from the spleen and lymph nodes from four different mice grafted with two thymic lobes for between 3 and 9 weeks.