Figure 6.

Recombination analysis in EAB cells. Site-specific recombination of the transfected V(D)J-containing plasmid pWTSJΔ results in transcription of the chloramphenicol-resistant gene and creates a new ApaLI restriction endonuclease site. (a) Recombination index (R.I.) of two EAB cell lines vs. a standard Abelson line, 204-1-8. %R.I. is the ratio of double-resistant Amp^R+Cat^R colonies over Amp^R colonies. (b) Inverted EtBr gel image of site-specific recombination products in the extrachromosomal V(D)J recombination assay, showing the two bands generated by a new ApaLI site.