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. 1999 Aug 17;96(17):9797–9802. doi: 10.1073/pnas.96.17.9797

Figure 7.

Figure 7

In vitro generation of ES-derived mature functional B cells. (a) Flow cytometric analysis for forward-scatter (FSC) by side-scatter (SSC). (b) CD19 by CD45R of ES/OP9 coculture with and without LPS treatment for 4 days. ELISA analysis of the culture supernatant collected 4 days after LPS stimulation showed high titer for secreted IgM (≈16 μg/ml). (c) Flow cytometric analysis for CD80 (B7-1) with and without LPS treatment for 48 hours. Mean fluorescence intensities: 10.25 for −LPS, and 21.81 for +LPS.