Table 1.
IL-1ra in the gastro-intestinal tract of mice inoculated intragastrically with recombinant S. gordonii GP1294.
| IL-1ra (OD450) at different time points after bacterial inoculuma | |||
| Sample b | (day 3) | (day 11) | (day 28) |
| Stomach | 0.478 | 0.244 | 0.000 |
| Small intestine | 0.061 | 0.062 | 0.000 |
| Caecum | 0.051 | 0.029 | 0.000 |
| Colon | 0.028 | 0.055 | 0.000 |
| Caecum content | 0.026 | 0.283 | 0.000 |
| Faeces | 0.036 | 0.025 | 0.000 |
a The presence of IL-1ra was determined by ELISA and expressed as net OD readings at 450 nm. As reference, standard IL-1ra readings were the following: 50 pg/ml, 0.018; 100 pg/ml, 0.030; 500 pg/ml, 0.236; 1000 pg/ml, 0.575. b IL-2-/- mice inoculated with the IL-1ra-expressing S. gordonii GP1294 strain were sacrificed 3, 11, and 28 days after the last inoculum. Different parts of the GI tract were removed, homogenised, and subjected to ELISA. Culture supernatants of strains GP1294 and GP204 were used as positive and negative controls, respectively.