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. 1999 Aug 17;96(17):9803–9808. doi: 10.1073/pnas.96.17.9803

Figure 2.

Figure 2

Impairment of CD27-mediated T cell costimulation but not in NF-κB or JNK/SAPK activation. (A) Thymocytes isolated from traf5+/+ (solid bars) and traf5−/−(hatched bars) mice were stimulated with anti-CD3 mAb in the presence of irradiated P815, P815-CD70, or P815-CD80 for 48 hr. Incorporation of [3H]thymidine was measured during the last 8 hr. Data are shown as mean cpm ± SEM from the triplicate samples and represent one of five independent experiments with similar results. (B) Thymocytes isolated from traf5+/+ and traf5−/− mice were stimulated with anti-CD27 followed by crosslinking with goat anti-hamster Igs for 15 min. EMSAs for NF-κB activation (Upper) and in vitro kinase assay for JNK/SAPK activation (Lower) were performed as described in Materials and Methods. B, oligonucleotide probe bound to NF-κB complexes.