Table 1.
In vitro Ig production of traf5+/+ and traf5−/− B cells
| IgM, ng/ml
|
IgG1, ng/ml
|
|||
|---|---|---|---|---|
| +/+ | −/− | +/+ | −/− | |
| Experiment 1 | 271.1 ± 40.0 | 66.7 ± 4.9 | 404.7 ± 21.6 | 130.0 ± 13.9 |
| Experiment 2 | 327.0 ± 34.1 | 137.3 ± 7.2 | 319.8 ± 19.3 | 222.5 ± 2.9 |
| Experiment 3 | 746.0 ± 217.6 | 104.7 ± 18.0 | 273.3 ± 28.0 | 80.7 ± 13.3 |
Purified B cells (1 × 105) were stimulated with anti-CD40 (10 μg/ml) plus IL-4 (10 ng/ml). After 6 days, IgM and IgG1 concentrations in the culture supernatants were measured by ELISA. IgM and IgG1 from unstimulated cultures were undetectable. Three independent experiments are shown, and the differences between traf5+/+ and traf5−/− are statistically significant (P < 0.001. Student’s t test).