Figure 6.

Time-dependent effects on Lucifer yellow dye transfer after exposure to lindane. A 1-h treatment of rat myometrial cells with 100 μM lindane initially abolished Lucifer yellow dye transfer (zero hour time-point), and then after removal of lindane exposure buffer and rinsing, this inhibition first reversed before redeveloping (ANOVA, p ≤ 0.0001). Mean values ± S.E.M. are shown (n = 3–5 culture dishes; 8–14 cells were injected per dish). Vertical bars with different letters have means that are significantly different from each other in pair-wise comparisons (Student-Newman-Keuls, p ≤ 0.05). Cultures exposed to solvent (DMSO) alone for 1 h were assessed 24 h after rinsing, and were found to transfer dye to 98.4 ± 1.7% of neighboring cells (data not shown on graph).