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aaTHEP1 is no NMP kinase. Autoradiography of thin-layer chromatograms showing samples containing [γ-32P]ATP after five minutes of incubation at 70°C and at different concentrations of ATP, AMP, GMP, and UMP, respectively. 1 μg of purified aaTHEP1 was used for each assay in 25 μl buffer.
