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Effect of BV13 on VE-cadherin internalization. Endothelial cells (1G11) were incubated with 50 μg/ml of BV13 for 1 hour and 7 hours (1 h and 7 h lanes) or nonimmune IgG for 7 hours (Control lane). Before extraction, endothelial cells were surface-labeled with biotin by using sulfo-nitrohydroxysuccinimido-biotin (16).
