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. 1999 Aug 17;96(17):9815–9820. doi: 10.1073/pnas.96.17.9815

Figure 7.

Figure 7

Complement requirements for BV13 effects on vascular permeability. BV13 was able to increase heart (white columns) and lung (striped columns) permeability in C5 complement-deficient animals (A). Mice DBA/2J (C5 deficient) and their matched controls C57BL/6N (Control) were injected with BV13 or the control mAb MEC 7.46 (100 μg/mouse), and permeability was evaluated after 7 hours. (B) Mice depleted of C3 complement. Animals were treated with either cobra venom factor as described in Materials and Methods to deplete C3 complement element (C3-depleted) or saline (Control). The mice then were injected i.v. with BV13 or MEC 7.46 (100 μg/mouse), and, after 7 hours, permeability in heart and lungs was measured. (C) Fab fragments of BV13 (200 μg/mouse) or BV13 whole mAb (100 μg/mouse) were injected i.v., and the organs were extracted after 2 hours. Data are expressed as percentage increase in Evans blue content of the different organs and are means ± SEM of three experiments, each performed in triplicates.