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. 1997 Jul 1;110(1):59–72. doi: 10.1085/jgp.110.1.59

Figure 6.

Figure 6

Effect of cross-linking on conductance and selectivity properties of double-cysteine mutants. (A) Raw current traces following depolarization to −10 mV from a holding potential of −120 mV for E403C/D1532C channels expressed in oocytes before (□) and after (▪) reduction with DTT. Note the nearly twofold increase in current after reduction at this voltage. (B) The corresponding current-voltage relationships for E403C/D1532C. In this particular mutant, there is little change in the channel's conductance (161 μS before and 156 μS after DTT), estimated from the slope of the current-voltage curve at voltages above 0 mV, but the reversal potential is shifted by 19 mV to the right following reduction with DTT. (C) Raw traces for E403C/ A1529C before (○) and after (•) the application of DTT. The current increased more than sixfold following reduction with DTT. (D) The current-voltage relationship for E403C/A1529C mutants shows a fivefold increase in slope at voltages above 0 mV (22 μS before and 97 μS after DTT), whereas the reversal potential is only slightly shifted rightward (7 mV) by reduction with DTT.