Table I.
Dissociation Constants (KD) for Cd2+ of WT and Mutant Na+ Channels before and after MTSEA Modification
| Mutants | IC50 (μM) | IC50 (μM) after MTSEA modification | ||
|---|---|---|---|---|
| WT (SkM1) | 1.8 ± 0.4 × 103 (3) | N/A | ||
| Y401C | 1.3 ± 0.3 (5) | 1.6 ± 0.1 × 103 (3) | ||
| W402C | 2.0 ± 0.1 × 102 (4) | 1.2 ± 0.1 × 103 (3) | ||
| E403C | 2.8 ± 0.2 × 102 (5) | 1.2 ± 0.2 × 103 (5) | ||
| W756C | 1.6 ± 0.1 × 103 (4) | 1.6 ± 0.4 × 103 (2) | ||
| I757C | 2.0 ± 0.2 × 102 (2) | 8.8 ± 0.7 × 102 (3) | ||
| E758C | 4.9 ± 0.3 × 102 (8) | 1.0 ± 0.1 × 103 (3) | ||
| G1238C | n.e. | n.e. | ||
| W1239C | 3.7 ± 1.0 × 10 (3) | 3.2 ± 0.3 × 102 (2) | ||
| M1240C | 5.7 ± 0.3 (5) | 1.2 ± 0.1 × 103 (2) | ||
| D1241C | 4.5 ± 0.5 × 102 (3) | 1.9 ± 0.3 × 103 (3) | ||
| A1529C | 3.0 ± 0.6 × 102 (3) | 8.0 ± 0.6 × 102 (2) | ||
| G1530C | 6.8 ± 0.9 × 10 (4) | 1.7 ± 0.2 × 103 (3) | ||
| W1531C | 4.6 ± 0.5 × 10 (5) | 1.6 ± 0.3 × 103 (6) | ||
| D1532C | 6.0 ± 0.7 × 102 (8) | 2.3 ± 0.3 × 103 (2) |
Numbers in parentheses represent number of determinations. n.e., no expression.