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The Changes in the Reversal Potential before and after Treatment with 10 mM DTT in Cross-linked Double-cysteine Mutant Channels
| Mutant | Erev (−DTT) | Erev (+DTT) | Observations | |||
|---|---|---|---|---|---|---|
| Wild-type | 55.7 ± 3.6 mV | 55.2 ± 2.9 mV | 6 | |||
| Y401C/E758C* | 36.9 ± 7.5 mV | 54.3 ± 8.5 mV | 3 | |||
| E403C/A1529C* | 31.1 ± 4.9 mV | 40.5 ± 4.2 mV | 4 | |||
| E403C/G1530C* | 11.7 ± 6.7 mV | 42.1 ± 7.4 mV | 3 | |||
| E403C/D1532C* | 12.6 ± 6.1 mV | 31.1 ± 6.6 mV | 5 |
Significantly different before and after DTT application (p < 0.05).
