Figure 7.
Mean steady state pHi increases when hippocampal astrocytes are switched from HEPES to CO2/HCO3 −. (A) Effect of CO2/HCO3 − on steady state pHi. During the indicated period, we switched to an extracellular solution that was buffered with 5% CO2/17 mM HCO3 − rather than HEPES (a–c). (B) The change in steady state pHi caused by adding CO2/HCO3 −, plotted as a function of steady state pHi in the HEPES-buffered solution.