Figure 7.

Current transients during forward and reverse GAT1 transport. (A) Outward GAT1 transport currents with 120 mM cytoplasmic NaCl, 120 mM extracellular Cl⁻, and no extracellular Na⁺. The solid line shows current activated by 20 mM cytoplasmic GABA, and the dotted line shows NO-711_i–defined current. (B) Inward GAT1 transport current with 0.4 mM extracellular GABA and 120 mM extracellular NaCl. Current records in the presence of 120 mM cytoplasmic Cl⁻(solid line) or 0.13 mM cytoplasmic NO-711 (dotted line) were subtracted from those with zero cytoplasmic substrates and zero inhibitor. Dashed lines represent zero current. Holding potential was 0 mV.