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Sidedness of aromatic TEA blocking site. TEA inhibition of KcsA channels substituted at position 82 was assessed as in Fig. 4. (A) cis TEA block. Solid curves are Langmuir fits with K i of 3.2, 23, and 143 for 82Y (▪), 82C (▴), and 82T (•) channels, respectively. (B) Trans TEA block. Solid curve is Langmuir function with K i = 25 mM.
