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MTSET modification of KcsA-Y82C. Single Y82C channel openings were recorded in symmetrical 100 mM K+. After recording under control conditions, MTSET (70 μM) was added to the cis chamber. Currents were monitored at negative potentials until an obvious change in conductance was observed (∼7 min). Unreacted MTSET was then removed by perfusion, and the “+MTSET” recordings were collected. (Top) Raw channel records. (Bottom) Open-channel I–V curves.
