Table 2.
Bacterial strains and plasmids used in this study.
| Strain | Genotype or description | Source or reference |
|---|---|---|
| CD43(DE3) | Avidis | |
| W3110 | Wild type, F-, λ- | E. coli Genetic Stock Center (Yale) |
| W3110A | Wild type, F-, λ-, aroA::Tn10 | Doerrler et al. (2001) |
| WD2 | W3110, aroA::Tn10 msbA (A270T) | Doerrler et al. (2001) |
| BW25113 | lacIqrrnBT14ΔlacZWJ16hsdR514ΔaraBADAH33ΔrhaBADLD78 | Datsenko and Wanner (2000) |
| BW25113A | BW25113 aroA::Tn10 | This work |
| BW2 | BW25113 aroA::Tn10 msbA (A270T) | This work |
| DMEG1 | BW25113 ΔbacA::CamR | El Ghachi et al. (2005) |
| DMEG2 | BW25113 ΔybjG::CamR | El Ghachi et al. (2005) |
| DMEG3 | BW25113 ΔlpxT::CamR | El Ghachi et al. (2005) |
| DMEG4 | BW25113 ΔpgpB::KanR | El Ghachi et al. (2005) |
| DMEG3/pTrcLpxT | BW25113 ΔlpxT::CamR/pTrcLpxT | This work |
| DMEG3/pTrcLpxT-EHEC | BW25113 ΔlpxT::CamR/pTrcLpxT-EHEC | This work |
| DMEG3/pTrcLpxT-ST | BW25113 ΔlpxT::CamR/pTrcLpxT-ST | This work |
| CD43/pLpxTHIS | CD43(DE3)/pLpxTHIS | This work |
| Plasmids | ||
| pTrc99A | Expression Vector containing a T7 promoter, AmpR | Amersham |
| pTrcLpxTEC | pTrc99A containing lpxT | El Ghachi et al. (2005) |
| pTrcLpxTEHEC | pTrc99A containing lpxT of E. coli O157:H7 | This work |
| pTrcLpxTST | pTrc99A containing lpxT of S. typhimurium LT2 | This work |
| pET2130 | Derivative of pET21d (Novagen) – expression vector, AmpR | El Ghachi et al. (2004) |
| pLpxTHIS | pET2130 containing lpxT | This work |
CamR and KanR represent inserted resistance genes to chloramphenicol and kanamycin respectively.